This is a list of cities with trolleybuses as part of their public transport system.

• Argentina
  • Córdoba
  • Mendoza
  • Rosario

• Armenia
  • Gyumri
  • Yerevan

• Austria
  • Innsbruck
  • Linz
  • Salzburg

• Azerbaijan
  • Baku
  • Ganca
  • Nakhchivan
  • Sumqayyt

• Belarus
  • Babruysk
  • Brest
  • Homel
  • Hrodna
  • Mahilyow
  • Minsk
  • Vitsebsk

• Belgium
  • Ghent

• Bosnia-Herzegovina
  • Sarajevo

• Brazil
  • Araraquara
  • Diadema
  • Recife
  • São Bernardo do Campo
  • São Paulo
  • Santo André

• Bulgaria
  • Burgas
  • Dobrich
  • Gabrovo
  • Khoskovo
  • Pazardzhik
  • Pernik
  • Pleven
  • Plovdiv
  • Ruse
  • Sliven
  • Sofia
  • Stara Zagora
  • Varna
  • Veliko Turnovo
  • Vratsa

• Canada
  • Edmonton, Alberta
  • Vancouver, British Columbia

• Chile
  • Valparaiso

• China
  • Beijing
  • chongqing
  • Dalian
  • Guangzhou
  • Hangzhou
  • Harbin
  • Jinan
  • Lanzhou
  • Luoyang
  • Nanchang
  • Nanning
  • Qingdao
  • Shanghai
  • Taiyuan
  • Wuhan
  • Xi'an
  • Zhengzhou

• Costa Rica
  • San José

• Czech Republic
  • Brno
  • České Budějovice
  • Chomutov - Jirkov
  • Hradec Králové
  • Jihlava
  • Liberec
  • Mariánské Lázně
  • Opava
  • Ostrava
  • Pardubice
  • Plzeň
  • Teplice
  • Ústí nad Labem
  • Zlín

• Ecuador
  • Quito (called trole, is fairly new)

• Estonia
  • Tallinn

• France
  • Limoges
  • Lyon
  • Marseille
  • Nancy
  • Saint-Etienne

• Georgia
  • Batumi
  • Chiatura
  • Gori
  • Kutaisi
  • Ozurgeti
  • Poti
  • Rustavi
  • Samtredia
  • Sokhomi
  • Tbilisi
  • Zugdidi

• Germany
  • Eberswalde
  • Esslingen
  • Solingen

• Greece
  • Athens

• Hungary
  • Budapest
  • Debrecen
  • Szeged

• Iran
  • Tehran

• Italy
  • Milan
  • Rome
  • Bologna
  • Ventimiglia

• Lithuania
  • Kaunas
  • Vilnius

• Mexico
  • Guadalajara
  • Mexico City

• The Netherlands
  • Arnhem

• New Zealand
  • Wellington

• North Korea
  • Chongjin
  • Hamhung
  • Kanggye
  • Pyongsong
  • Pyongyang

• Norway
  • Bergen

• Poland
  • Gdynia
  • Lublin
  • Tychy

• Romania
  • Bucharest
  • Constanţa
  • Cluj-Napoca
  • Iaşi
  • Târgovişte
  • Timişoara

• Russia

The most of the cities with population more than 200'000 have trolleybus

• • Cheboksary
  • Irkutsk
  • Ioshkar-Ola
  • Kazan
  • Kaliningrad
  • Kirov
  • Moscow
  • Murmansk
  • Naberezhnye Chelny
  • Saint Petersburg
  • Vladivostok
  • Ulyanovsk
  • Ufa
• Serbia
  • Belgrade

• Slovakia
  • Banska Bystrica
  • Bratislava
  • Kosice
  • Zilina

• Sweden
  • Landskrona

• Switzerland
  • Basel (there are plans of withdrawal)
  • Bern
  • Biel/Bienne
  • Fribourg
  • Geneva
  • La Chaux-de-Fonds
  • Lausanne
  • Lucerne
  • Neuchâtel
  • Schaffhausen
  • St. Gallen
  • Vevey-Montreux-Villeneuve
  • Winterthur
  • Zürich

• Ukraine
  • Alchevsk
  • Alushta
  • Antracyt
  • Artemivsk
  • Bila Cerkva
  • Cherkasy
  • Chernihiv
  • Chernivci
  • Dnipropetrovsk
  • Dobropilla
  • Donetsk
  • Dzerzhynsk
  • Horlivka
  • Ivano-Frankivsk
  • Kerch
  • Kharcyzk
  • Kharkiv
  • Kherson
  • Khmelnycky
  • Kirovohrad
  • Kramatorsk
  • Krasnodon
  • Kremenchuk
  • Kryvy Rih
  • Kyiv
  • Luhansk
  • Luck
  • Lysychansk
  • Lviv
  • Makiivka
  • Mariupol
  • Mykolaiv
  • Odessa
  • Poltava
  • Rivne
  • Sevastopol
  • Severodonetsk
  • Simferopol-Yalta (Crimea, world's longest line, 117 km)
  • Slovjansk
  • Stakhanov
  • Sumy
  • Ternopil
  • Vinnycia
  • Vuhlehirsk
  • Zaporizha
  • Zhytomyr

• United States
  • Boston, Massachusetts's Silver Line (new trolleybuses delivered 2004) and routes near Harvard Square in Cambridge, Massachusetts
  • Dayton, Ohio
  • Philadelphia, Pennsylvania (suspended for one year starting June 2003)
  • San Francisco, California (operated by San Francisco Municipal Railway)
  • Seattle, Washington