Melchior can refer to

• One of the Three Wise Men
• In the anime series Neon Genesis Evangelion, one of three parts of the biocomputer located in NERV's First Branch.
• A fan-made game of the "shoot em' up" genre for the Sega Dreamcast started as a school project by three people from Sweden
• Lauritz Lebrecht Hommel Melchior (1890–1973) was a Danish-born operatic tenor noted for his Wagnerian roles
• The Guru of Life in the game Chrono Trigger.