The acronym NLS has several possible meanings:

• In biology, NLS stands for nuclear localization signal.
• NLS was the name of a pioneering computer system: see NLS (computer system)
• In computing, NLS also stands for National Language Support .
  • It also stands for Native Language Support , a system developed by Hewlett-Packard for software internationalization, though many think that "N" means "national" here, too.
• NLS also stands for National Lifeguard Service in Canada.